Autor: de Jong, F.; Pokorny, J.; Manshian, B.; Daelemans, B.; Vandaele, J.; Startek, J. B.; Soenen, S.; Van der Auweraer, M.; Dehaen, W.; Rocha, S.; Silveira-Dorta, G.
Název práce v češtině: Development and Characterization of BODIPY-Derived Tracers for Fluorescent Labeling of the Endoplasmic Reticulum
Název práce v angličtině: Development and Characterization of BODIPY-Derived Tracers for Fluorescent Labeling of the Endoplasmic Reticulum
Studentská publikace: ne
Klíčová slova v češtině: –
Klíčová slova v angličtině: –
Abstrakt česky: –
Abstrakt anglicky: Visualization of the structure of the Endoplasmic Reticulum (ER) in living cells is important for the understanding of its function. Here we synthesized a library of 8 BODIPY labeledhydroxyquinoline derivatives and evaluated their spectroscopic properties, cytotoxicity, and intracellular localization. The compounds were easily obtained in 34–80% yield. Based on the spectral properties and low cytotoxicity, we selected the quinolin-8-yl pentanoate derivative 17 for in vivo labeling of the ER. Fluorescence staining of the ER was evaluated by comparison with a commercial ER tracker. The molecules here developed are smaller than the alternatives commercially available while still presenting a high specificity towards the ER.
Jazyk v originále: EN
Název časopisu: Dyes and Pigments
Rok: 2020
Svazek (ročník): 176
Číslo časopisu v rámci uvedeného svazku: –
Strana od-do: 108200
Impact factor: 4
Q1: ne

ISSN časopisu: 0143-7208
Vydavatel: Elsevier
Způsob financování: –
DOI: 10.1016/j.dyepig.2020.108200