Autor: Motyka, K; Brulíková, L.; Labora, M.; Hlaváč, J.
Název práce v češtině: Nové fluorescenční rhodaminové systémy vyvinuté pomocí kombinatoriálního přístupu aplikací syntézy na pevné fázi
Název práce v angličtině: Novel rhodamine based fluorescent systems developed by combinatorial approach with application of solid-phase synthesis
Klíčová slova v češtině: rhodamin, duální emise, syntéza na pevné fázi, dusitany, pH
Klíčová slova v angličtině:rhodamine, dual emission, solid-phase, nitrite, pH
Abstrakt česky: V práci je popsán vývoj fluorescenčních systému založených na rhodaminové struktuře. K problematice bylo přistupováno kombinatoriálním způsobem s aplikací syntézy na pevné fázi.
Abstrakt anglicky: Fluorescence probes based on rhodamine structure as a fluorescent dye gain in importance in last years, especially in studies of biological systems (monitoring of intercellular pH1, DNA studies2, the detection of specific proteins3 and more). For synthesis of novel rhodamine based fluorescent systems a combinatorial approach with application of solid-phase synthesis was utilized. The precise design of the appropriate fluorescent system is difficult to set up and therefore several similar compounds have to be synthesized. For this reason high-throughput methods for preparation of chemical libraries for selection of suitable candidates for further development of new fluorescent systems are being developed. The fluorescent systems consisted of rhodamine as the fluorescent dye, linker and central group (formed by 4,6-dichloro-5-nitropyrimidine that bears the possibility to bind three different substituents). The structure changes of central part can be predicted to have different excitation/emission properties. Prepared compounds was evaluated for their fluorescence properties and some interesting results was obtained. The various substitution of central group as well as different linker affect especially the fluorescence intensity (the fluorescence quantum yields move from 0.1 to 69 %). The excitation and emission spectra of prepared rhodamine based fluorescent systems mostly show similar shape and maxima as rhodamine B (excitation and emission maxima move in the range from 550 to 570 nm and 572 to 592 nm, respectively). Besides this fact some fluorescent systems exhibit a dual emission spectra if lower excitation wavelength is applied. Although the fluorescence quantum yields are significantly lower (more than 200 times), the fluorescence intensity of the dual emission is still sufficient and, moreover, offers next possibilities. In the case of the common single-band fluorescent labels the fluorescence intensity depends on the label concentration that can vary because of various biological processes in a sample. A dual fluorescence labels that exhibit two well-separated emission bands are not dependent on the concentration because the ratio of the intensities of the two bands can be applied as a signal4,5. For one derivative it was found out that the dependence of the ratio of fluorescence intensities at local maxima on pH is linear and the fluorescent system can be utilized as pH indicator. Further, the dependence of maxima ratio on nitrite concentration in aqueous solution was observed as well.
Jazyk v originále: angličtina
Publikace abstraktu: Janov, Itálie
Forma prezentace: poster
Název konference: 13th Conference on Methods and Applications of Fluorescence
Místo konání: Janov (Itálie)
Datum konání: September 8 – September 11, 2013
Rok konání: 2013
Způsob financování: –