Autor: Olson, A. C.; Patro, N. J.; Urban, M.; Kuchta, R. D.
Název práce v češtině: Energeticky rozdil mezi syntezou spravneho a nespravneho paru bazi je zodpovedny za velmi presnou replikaci DNA.
Název práce v angličtině: The Energetic Difference between Synthesis of Correct and Incorrect Base Pairs Accounts for Highly Accurate DNA Replication
Studentská publikace: ne

Klíčová slova v angličtině: DNA replication, Energetics, Base pairs.
Abstrakt česky: Abychom lepe porozumeli energetice presne DNA replikace, primo jsme zmerili ΔGo inkorporace nukleotidu do prodluzujici se dvousroubovice DNA v roztoku (ΔGo incorporation). Prima mereni energetickeho rozdilu mezi zabudovanim spravneho nukleotidu a nespravneho ukazala, ze rozdil je mnohem vetsi, nez se v minulosti predpokladalo (prumerny ΔΔGo incorporation = 5.2 ± 1.34 kcal mol−1). Je dulezite, ze tato prima mereni ukazuji, ze i sama energeticka preference vzniku spravneho paru muze byt zodpovedna za vysokou presnost replikace DNA. Z evolucniho hlediska to idikuje, ze prvotni polymerazy nemusely vyvijet slozite mechanizmy, aby zajistily vysokou presnost DNA replikace a mohly pouze vyuzit vyhody tohoto velkeho energetickeho rozdilu. Tyto vysledky prinaseji zakladni platformu pro porozumeni, jakym zpusobem je zajistena vysoka presnost replikace DNA pomoci DNA polymeraz.
Abstrakt anglicky: To better understand the energetics of accurate DNA replication, we directly measured ΔGo for the incorporation of a nucleotide into elongating dsDNA in solution (ΔGo incorporation). Direct measurements of the energetic difference between synthesis of correct and incorrect base pairs found it to be much larger than previously believed (average ΔΔGo incorporation = 5.2 ± 1.34 kcal mol−1). Importantly, these direct measurements indicate that ΔΔGo incorporation alone can account for the energy required for highly accurate DNA replication. Evolutionarily, these results indicate that the earliest polymerases did not have to evolve sophisticated mechanisms to replicate nucleic acids; they may only have had to take advantage of the inherently more favorable ΔGo for polymerization of correct nucleotides. These results also provide a basis for understanding how polymerases replicate DNA (or RNA) with high fidelity.
Jazyk v originále: Anglicky
Název časopisu: J. Am. Chem. Soc.
Rok: 2013
Svazek (ročník): 135
Číslo časopisu v rámci uvedeného svazku: 4
Strana od-do: 1205-1208

Q1: ne

Způsob financování: NIH AI59764