Autor: Müller, L.; Barták, P.; Bednář, P.; Fryšová, I.; Ševčík, J.; Lemr, K.
Název práce v češtině: Capillary electrophoresis-mass spectrometry – a fast and reliable tool for the monitoring of milk adulteration
Název práce v angličtině: Capillary electrophoresis-mass spectrometry – a fast and reliable tool for the monitoring of milk adulteration
Studentská publikace: ne

Klíčová slova v angličtině: Food analysis • -Lactoglobulins • Milk • Proteins
Abstrakt česky: The development of a rapid, simple and accurate analytical method aimed at the detection and quantification of bovine milk in either ovine or caprine milk samples by means of CE-MS analyses of whey proteins with high-ionic strength and presence of acidic running buffer is described. The high-ionic strength buffer was used in order to minimize the problems with the adsorption of the proteins onto the fused-silica capillary wall. The acidic running electrolyte, pH 1.9, was used to support the production of positive ions in electrospray. Highly linear dependences of the ratio of the sum of non-bovine -lactoglobulins (ovine or caprine) to the total -lactoglobulins in milk mixture (bovine plus ovine or bovine plus caprine) vs. the volume percentage of added bovine milk in ovine (or caprine) milk were obtained. This technique allowed the fast and reliable evaluation of milk adulteration. The amount of bovine milk added into the non-bovine ones can be well within the concentration range of 5-95%.
Abstrakt anglicky: The development of a rapid, simple and accurate analytical method aimed at the detection and quantification of bovine milk in either ovine or caprine milk samples by means of CE-MS analyses of whey proteins with high-ionic strength and presence of acidic running buffer is described. The high-ionic strength buffer was used in order to minimize the problems with the adsorption of the proteins onto the fused-silica capillary wall. The acidic running electrolyte, pH 1.9, was used to support the production of positive ions in electrospray. Highly linear dependences of the ratio of the sum of non-bovine -lactoglobulins (ovine or caprine) to the total -lactoglobulins in milk mixture (bovine plus ovine or bovine plus caprine) vs. the volume percentage of added bovine milk in ovine (or caprine) milk were obtained. This technique allowed the fast and reliable evaluation of milk adulteration. The amount of bovine milk added into the non-bovine ones can be well within the concentration range of 5-95%.
Jazyk v originále: anglický
Název časopisu: Electrophoresis
Rok: 2008
Svazek (ročník): 29
Číslo časopisu v rámci uvedeného svazku: 10
Strana od-do: 2088-2093

Q1: ne

Způsob financování: MŠMT