Autor: Jirovský, D.; Kosina, P.; Myslínová, M.; Stýskala, J.; Ulrichová ,J.; Šimánek, V.;
Název práce v češtině: HPLC analýza osmarinové kyseliny v krmivu obohecené nadzemní části rostliny Prunella vulgaris a jejch metabolitů v pracečí plazmě metodou dvoukanalové kulometrické titrace
Název práce v angličtině: HPLC Analysis of osmarinic Acid in Feed Enriched with Aerial Parts of Prunella vulgaris and Its Metabolites in Pig Plasma Using Dual-Channel Coulometric Detection
Studentská publikace: ne

Klíčová slova v angličtině: coulometric detection; rosmarinic acid; pigs; metabolism; feed
Abstrakt česky: Tato práce popisuje citlivou isokratickou HPLC/ECD metodu vyvinutou pro stanovení rosmarinové kyseliny v rostlinných materiálech, krmiv pro hospodářská zvířata a prasečí plasmu.
Abstrakt anglicky: This paper describes a sensitive isocratic HPLC/ECD method developed for the determination ofrosmarinic acid (RA) in plant material, animal feed, and pig plasma. The plasma sample preparation only includes protein precipitation and adjustment of the pH. The applicability of the method was tested on plasma samples of pigs that were exposed to a 91-day oral intake of RA via feed enriched by aerial parts of Prunella vulgaris. The plasma was directly analyzed using the method described as well as after enzymatic hydrolysis. When no hydrolysis step was included, RA and caffeic acid(CA) were quantified in the plasma. In hydrolyzed plasma samples, several other metabolites were determined, including dihydrocaffeic, ferulic, and dihydroferulic acid. The dual-channel coulometric detection employed, as an alternative to mass spectrometry, offers good selectivity and sensitivity owing to the electrochemical properties of the phenolic constituents.““
Jazyk v originále: Angličtina
Název časopisu: J. Agric. Food Chem.
Rok: 2007
Svazek (ročník): 55

Strana od-do: 7631-7637

Q1: ne

Způsob financování: MSM6198959216