Course Guarantor

Jan Hlaváč

Form of course completion:

Exam

Annotation:

1. Fluorescence basis

• Fluorescence principles
• Excitation/emission spektra, anisotropy, Stokes shift, quantum yield
• Instrumentation

2. Fluorophores

• Fluorophores in living systems
• Extrinsic fluorophores for biomolecules labelling
• Quantum dots
• Metal complexes

3. Factors affecting the fluorescence

• Solvent effects
• Other factors affecting the fluorescence

4. Fluorescence quenching

• Mechanism of fluorescence quenching
• Examples and applications of fluorescence quenching

5. Fluorescence energy transfer (FET)

• Principles and conditions of FET
• Transfer effectivity
• Examples and practical applications

6. Fluorescent proteins

• Aminoacids and proteins with fluorescent properties
• Protein structure and fluorescence
• Fluorescent proteins in practical application

7. Fluorescent probes

• Intensiometric and ratiometric probes –comparison, examples
• Multifunctional probes – examples and principles

8. Application of fluorescence in molecular

• Logic gates based on fluorescence
• Application of molecular electronics devices for detection

Literature:

• Lakowicz, J.R. Principles of fluorescent spectroscopy; 3rd edition; Springer; 2006; ISBN: 978-0-378-31287-1
• Albani, J.R. Principles and applications of fluorescent spectroscopy; Wiley-Blackwell|; 2007; ISBN: 978-1-405-13891-8
• Nelson, D. L., Cox, M. M. Lehninger Principles of Biochemistry 6th edition, Macmillan
• Original papers relevant to the topic.